标签归档:CRISPR-Cas9

2016
01-19
High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects Abstract CRISPR–Cas9 nucleases are widely used for genome edit.... Read More >
2015
07-06
漫话基因组编辑之三:CRISPR-Cas9的羁绊-脱靶效应   以CRISPR-Cas9为代表的基因组编辑技术火热一时,小编特地精心准备了漫话基因组编辑系列三部曲,以飨读者。今天给大家献上最终曲,CRISPR-Cas9的羁绊-脱靶效应。 “改造人”的争论点 上一期已经说.... Read More >
2015
03-24
Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining Abstract Methods to introduce targeted double-strand breaks (DSBs) into DNA enable.... Read More >
2015
03-16
笔者注:基因编辑技术的快速发展为疾病的诊断和治疗提供了新的灵感,对于白血病这种癌症可以编辑有问题的造血干细胞,可以筛查编辑有疾病风险的胚胎,对于成人遗传病,可以编辑IPS细胞。不过,在这些应用之前,保证基因编辑的安全性首当其冲,利用WGS高深度测序检测offtarget效应很必要。 3月5日,.... Read More >
2015
03-13
Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells Abstract Although RNA-guided genome editing via the CRISPR-Ca.... Read More >
2015
02-27
Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids Human colorectal tumors bear recurrent mutations in genes encoding proteins operative in the WNT, MAPK,.... Read More >